By direct method of isolation, total seven potent yeast isolates were obtained namely CP1, CP2, B2X1, B2X2, BE, GE1 and GE2, which were further screened for their better utility. Out of this, best ethanol producer yeast strain BE was further studied for the optimization of different physical and chemical parameters. Maximum ethanol production obtained at pH-7 while optimum xylose concentration for highest ethanol production was found to be 2%. Best economically viable nitrogen source found to be casamino acid. The same isolate was also found to carry out ethanol fermentation with different sugar source and showed significant results. | Isolation and characterization of xylose fermenting yeast from different fruits for bioethanol production