The new challenge of irrigated lowland rice is to sustain the needed increase in rice yields while reducing the requirements for water and N per unit of grain production. Validation of markers helps in determining the reliability and practical applicability of the markers in predicting the phenotype. In the present study, Simple sequence repeats (SSR) markers flanking WUE and NUE traits reported to explain high percentage phenotypic variation for their respective traits in RILs population from a cross Swarna x WAB450 were used for validation in F2 population individuals of BPT5204 x WAB450 and BPT5204 x Mysore Mallige crosses for the same traits for which they are already reported to be associated. Single marker analysis was done to with the help of student’s t test and by single factor ANOVA. | Marker validation in F2 population of rice (Oryza sativa L.) for water and nitrogen use efficiency
