In the present study extracellular polysaccharide (EPS) was characterized from Pseudomonas aeruginosa B01. EPS was purified using ethanol precipitation and dialysis. The purified EPS from P. aeruginosa B01 was analyzed using a High Performance Liquid Chromatography (HPLC). The infrared spectra of EPS from P. aeruginosa B01 clearly showed the presence of carboxylate groups. The FT-IR spectrum indicated an intense absorption band in the region of 3296 cm-1 on the stretching vibration of hydroxyl groups (OH). The bands in the region of 2941 cm-1 and 1627 cm-1 are due to stretching vibrations of C-H bonds. The absorption in the region of cm-1 indicated the presence of glycoside link in the alpha (α) conformation. | Isolation and characterization of larvicidal extracellular polysaccharide (EPS) from Pseudomonas aeruginosa B01