microsatellite markers Tomato (Solanum lycopersicum L.) is one of the most important economically plants in the family Solanaceae. Information about genetic diversity of any crop is important for successful employment of breeding programme and is of great significance to attain sustainability in crop production. The objective of this study was to evaluate the genetic diversity of 24 genotypes of tomato collected from ICAR-IIVR, Varanasi, India, using 25 simple-sequence repeats (SSRs). A total of 64 alleles were detected at an average of alleles per SSR locus. The average major allele frequency and polymorphic information content were and , respectively. | Evaluation of genetic variability in tomato (Solanum lycopersicum L. Mill) genotypes using microsatellite markers