Staphylococcus spp is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system. Establishment of a mature biofilm, play an important role in the persistence of chronic infections and also decreases the susceptibility to antimicrobials and immune defenses, making these infections difficult to eradicate. MRSA and MR-CONS has become a major public health problem in both hospitals and communities. PVL has been reported to be an important marker for the highly pathogenic community acquired S. aureus infections. | Prevalence of MRSA and biofilm associated gene among clinical isolates of staphylococci