Trong nghiên cứu này tiến hành xác định biểu hiện mRNA của các gen này trên bệnh nhân THCVC bằng kỹ thuật realtime-PCR và nồng độ CA125 bằng kỹ thuật ELISA. Kết quả cho thấy, biểu hiện các gen klotho, LAG3, CTLA-4 và PD-1 giảm xuống ở bệnh nhân THCVC chỉ ra hệ miễn dịch đối kháng chưa hoạt động. Nồng độ CA125 tăng cao rõ rệt so với người khỏe và 3 bệnh nhân có nồng độ CA125 tăng tỷ lệ thuận với chỉ số hóa sinh LDH, AST và ALT trong máu người bệnh. | Biểu hiện một số gen kiểm soát miễn dịch và nồng độ CA125 ở bệnh nhân tăng hồng cầu vô căn TAP CHI SINH HOC 2020, 42(1): 103–110 DOI: GENE EXPRESSIONS INVOLVED IN IMMUNE SYSTEM CONTROL AND SERUM CA125 CONTENT IN POLYCYTHEMIA VERA Do Thi Trang1, Nguyen Thi Xuan1,2,* 1 Institute of Genome Research, VAST, Vietnam 2 Graduate University of Science and Technology, VAST, Vietnam Received 9 December 2019, accepted 4 March 2020 ABSTRACT Polycythemia Vera (PV) is a slowly progressing blood cancer associated with myeloproliferative neoplasms. The disease is characterized by an abnormal proliferation of three cell types including red blood cells, white blood cells and platelets and a symptom of pruritus caused by release of itching agents of activated mast cells. The enhanced expression of several genes involved in immune system control including CTLA-4, PD-1 and LAG3 are linked to activation immune tolerance. Klotho gene has anti-aging, anti-inflammation and anti-cancer functions. The SHP gene group belongs to the tyrosine phosphatase protein signaling family that regulates cancer cell proliferation through maturation, migration and apoptosis and includes two main genes, SHP-1 and SHP-2. The increased serum content of cancer antigen CA125 is considered as a cancer marker of several blood and hematopoietic disorders. In this study, we conducted experiments to determine mRNA expression of above genes in PV patients by realtime-PCR and CA125 concentration by ELISA. Results showed that expression of klotho, LAG3, CTLA-4 and PD-1 genes was decreased in PV patients, indicating that the immune tolerance was inactivated in PV patients. CA125 concentration was significantly increased in PV patients compared to healthy individuals and interestingly, there was a positive association among three patients, who having increased CA125 concentration and biochemical indicators including LDH,
