Vitamin B6, also called pyridoxine, is one of the B vitamins. It helps the body to convert food (carbohydrates) into fuel (glucose), which is used to produce energy. A simple and sensitive reversed-phase (RP) HPLC method was used for determination of vitamin B6 present in areca nut samples. Areca nut samples were collected from Shimoga, Davanagere, Chikkamagalur, Chitradurga, Dakshina Kannada and Udupi districts of Karnataka, India. RP- HPLC analysis was performed with UHPLC system (Thermo fisherultimate 3000) equipped with UV/Visible detector and C18 column. | Techniques for determination of vitamin B6, vitamin C and variability in Areca nut (Areca catechu) samples of Karnataka, India