Nowadays, there are many different procedures for the laboratory diagnosis of Toxoplasma infection in pregnant women, congenitally-infected fetuses and newborns, and they are mainly performed by serological testing, and PCR (for confirmatory purpose), beside many commercial diagnostic tests, which use Toxoplasma lysate antigen. These procedures differ from each other in many aspects; cost, time-consumption, and accuracy of the test, which should meet patient’s needs. | Diagnosis of human toxoplasmosis using rapid chromatographic immunoassay and enzyme-linked immuno-sorbent assay (ELISA) compared to molecular technique (PCR) as gold standard technique