Banana is one of the most important and remunerative cash crops grown round the year in Ethiopia. The energy and nutritional status of banana are much higher than other common tropical and subtropical fruits. The objective of this study was to establish in vitro root development protocol in Poyo and Grand naine varieties from sucker. For root induction suckers of Poyo and Grand naine were cultured on MS basal medium that contained 30 g/l sucrose, 6 g/l agar and vitamins. | In vitro root development system in two popular cultivars of banana