PsbO is a ubiquitous extrinsic protein of photosystem II required for providing proper environment for water splitting activity of photosystem II. Exposure of wheat to heat stress initially reduces the rate of photosynthesis by disintegrating the photosystem II, primarily by disruption of the oxygen evolving complex (OEC). Here, we identified and cloned putative PsbOgene of 1 kb from wheat cv. HD2329 using de novo assembly. Expression analysis showed higher expression of PsbO transcript in HD2985, as compared to HD2329 during pollination and grain-filling stages. | Wheat oxygen evolving enhancer protein: Identification and characterization of mn-binding metalloprotein of photosynthetic pathway involved in regulating photosytem II integrity and network of antioxidant enzymes under Heat stress