The aim of this study was to characterize the antimicrobial peptide (bacteriocin) produced by a probiotic Lactobacillus plantarum strain DM 69 isolated from yogurt sample of Odisha. Total DNA of L. plantarum DM 69 was extracted and tested for the presence of bacteriocin genes. PCRamplified DNA showed a single band in gel which partially confirmed the presence of Plantaricin gene responsible for showing antimicrobial activity. The purified antimicrobial peptide was found to be stable at 60 0C, 80 0C up to 60 min and 100 0C for 30 min, pH 2-8, 1-8% NaCl and in presence of digestive enzymes such as α-Amylase, trypsin, lipase and lysozyme. | Characterization of antimicrobial peptide produced by probiotic lactobacillus plantarum DM 69 isolated from yogurt sample of Odisha, India
