Fluorescence microscopy allows an extensive variety of analyzes in structural biology. Until the beginning of the seventeenth century living beings were limited to macroscopic organisms only, and there was no knowledge of the microscopic universe. Epi-fluorescence and Confocal microscopy appears in 1995 by Marvin Minsky with the aim of increasing the contrast and constructing three-dimensional images by the use of an aperture, allowing a great definition. | Cell membrane damage of prokaryotes and analysis of the cell death type in eukaryotes by fluorescence microscopy
