Present study aimed to screen commercial milk and milk products sold in the local markets of Bhubaneswar, Odisha for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). A total of seventeen (17) samples comprising of commercial milk and milk products (curd, flavoured milk, lassi and milk powder) of leading commercial brands were purchased from the local markets in Bhubaneswar, Odisha. Samples were tested using antigen detection tests [milk microscopy (ZN staining), IS900 PCR, IS900 Real Time PCR, Indirect Fluorescent Antibody Test (iFAT)] and antibody detection test [Indigenous plate ELISA test (i_ELISA) by indigenous ELISA kit (provided by Animal Health Division of CIRG, Makhdoom)] in the Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom. None (0%) of the 17 samples tested positive for MAP by any of above the tests, however, 12 were suspected for MAP by i_ELISA. The results indicated that the presence of MAP infection was very low, which sometimes becomes difficult to detect by multiple tests due to less sample size. | Bio-detection of mycobacterium avium subspecies paratuberculosis in the commercial milk and milk products sold in Bhubaneswar, Odisha, India
