The simple sequence repeat (SSR) or microsatellite marker is currently the most preferred molecular marker because of its reproducibility and codominant nature. The aim of this study was to validate SSR markers for bacterial wilt (BW) and tomato leaf curl virus disease (ToLCV) in tomato. DNA isolated from the parents Mukthi and IIHR-2195 was used to validate five SSR primers already reported for BW and ToLCV. One primer SSR20 which showed good polymorphism and reproducibility among parents were selected for further validation in F3 and F4 population. SSR20 was validated on resistant F4, their corresponding F3 parental lines, along with susceptible checks. The SSR20 segregated with the trait in the F3 and F4 resistant plants and was also found expressed in few susceptible checks. SSR20 identified in the study could be utilized for marker assisted selection with respect to bacterial wilt in tomato. | Validation of SSR markers for imparting disease resistance in tomato (Solanum lycopersicum L.)
