The present study comprised of three male sterile CMS lines, three maintainer lines and ten male fertile males (testers/ restorers) with three standard checks in two sets . CMS and conventional thus making 60 F1s (30 CMS and 30 conventional) using Line x Tester mating design. The crosses, CAK 53A x AKH- 07R, CAK 23B x AKH-07R, CAK 23B x DHy286-1R, CAK 23B x R-2000-23 and SRT-1A x R-2000-26 were found better for seed cotton yield per plant and hold promise for further evaluation and commercial exploitation of heterosis both in CMS and conventional system for high yield. | Heterosis studies in CMS based and conventional hybrids for yield and yield contributing traits in cotton (G. hirsutum L.) over the environments