L-asparaginase acts as an efficient agent in curing certain sorts of lymphoma and leukemia by catalyzing the deamination of L-asparagine to L-aspartate and ammonia. Microorganisms are better source of L-asparginase, as their culturing, extraction and purification is more convenient than plants and other sources. As most of L-asparginases are intracellular in nature, so the selection of a suitable method for its release with maximum recovery was become more important. In present study, the resting cells of S. marcescens MTCC 97 were disintegrated by different enzymatic (lysozyme), chemical (alkali lysis, acetone powder, guanidineHCl and triton X-100) and physical (motor and pestle, vortex, bead beater and sonicator) methods. Among all methods explored, sonication was found best method with U/mg specific activity and minimum loss of enzyme (8%). Different reaction parameters were also optimized for the characterization of released L-asparginase. | Optimization of extraction techniques for the release of intracellular L-asparginase from serratia marcescens MTCC 97 and its characterization
