Cold active lipase was investigated by bacteria and yeast isolates from the core sample of Nella Lake, Larsemann Hills region, East Antarctica. Among potential yeasts and bacteria producing lipases, best isolates were identified as Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 by molecular technique. In order to enhance the lipase production capabilities, both the isolate again subjected for optimization processes using various physiological (temperature and pH) and chemical (Carbon, nitrogen, minerals and various substrates like oils and triglycerides) parameters. | Cold active lipases produced by cryptococcus sp. Y-32 and rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica