A total of 368 dogs brought to the Teaching Veterinary Clinical Complex, Guwahati, Assam, India were screened for tuberculosis using tuberculin skin test (TST) technique and out of which a total of 6 dogs each from both the reactor and non-reactor group were included for the study. Hemogram of tuberculin positive reactors revealed significant (P≤) reduction in Hb, PCV, TEC, TLC, neutrophils and MCHC when compared to tuberculin negative reactors. Alterations in biochemical parameters included significant (P≤) increase in serum Aspartate transaminase (AST), Alanine transaminase (ALT) and Alkaline phosphatase (ALP) and serum calcium concentrations and reduced serum albumin and inorganic phosphorous.