The present study is based on the characterization of the protease producing bacteria from the soil present outside and adjacent areas of a sweetshop in Burdwan. Screening of the bacteria was done by employing skim milk agar plating technique at 37°C and observed after 24 hours. Proteolytic activity of the crude protease extract from the bacteria was detected by using azocasein as substrate. Two bacteria was initially screened; one was giving white colony and the other was giving yellow colony. White colony bacteria showed to give higher activity. | Isolation of protease producing bacteria from soil and characterization of the protease