Effective surface sterilization and shoot regeneration protocols for Clinacanthus nutans are described. Nodal segments of C. nutans were sterilised by single- and double-stage sterilisation methods. In the double-stage method, explants were soaked in Plant Preservative Mixture (PPM) at various concentrations (0, , , µL/mL). | Effective surface sterilization method using plant preservatives mixture and shoot multiplication of Clinacanthus nutans