Ethanol production is also known as ethanolic fermentation in which sugars of biomaterials are converted into the ethanol and carbon hydroxide that may called as coproduct of fermentation. In general fermentation is a bio-chemical process where decomposition of biomaterials takes place. During bio-chemical reduction the sugar compounds like sucrose, fructose, glucose and lactose are converted into the ethyl ethanol and CO2 as by product of the fermentation process. The yield of ethanol greatly depends upon the amount of sugar content, conversion rate (fermentation rate), type of culture and aerobic and anaerobic condition. | Effect of different pre-treatment methods on reducing sugar of rice substrate to enhance the ethanol yield