The Vertical distribution of Collembola in different soil layers namely, organic layer (O), 0-5 cm soil depth and 5-10 cm soil depth were studied. The majority of Collembola were recorded from the ‘O’ layer and upper layer in all the season except some exception in hot summer. During summer season, vertical migration of Collembola were seen whereas, such trend is very least seen during winter season where sufficient soil humidity was maintained. Same pattern was seen at species level also expected certain thermopilic species. The study also revealed that smaller species inhabit deeper layers. | Vertical distribution of collembola (Arthropoda: Collembola) at Varanasi, India