Chilli (Capsicum annuum) is an important spice as well as vegetable crop that are grown throughout the world especially in tropical and subtropical regions. India is a major producer, exporter and consumer of chilli. Chilli is grown in almost all states. Anthracnose disease can occur on leaves, stems, and both pre and post-harvest fruits. It is one of the major and devastating diseases of chilli causes severe losses (10-60%) both in yield and quality of the chilli depending upon the varieties. Diseases are the major problem for successful cultivation of chilli in India. For successful cultivation of chilli, it is important to identify resistant and tolerant varieties against anthracnose diseases. Therefore, experiment was conducted in the Department of Plant Pathology, college of Agriculture Gwalior and the field experiment were carried out in the Krishi Vigyan Kendra Research farm, RVSKVV Gwalior (.) using CRD with 3 replication during kharif 2015-16. For Screening of 25 varieties & genotypes of chilli received from ICAR/National institutes were evaluated against anthracnose (Colletotricum capsici) under laboratory condition using three artificial inoculation treatment viz distilled water inoculation, without injury inoculation, injury + inoculation. Under laboratory/controlled condition, Semiripe full mature chilli fruits (25 days old) were chosen for this study. Fruits were carefully detached from plants and washed with sterile distilled water (SDW). | Screening of different chilli genotypes against anthracnose disease (Colletotrichum capsici) under controlled condition