For improvement of genetic program in animal, artificial insemination (AI) with liquid or frozen semen and quality of semen plays a major role for it. Sperm cells have a high content of polyunsaturated fatty acids in their membranes, and they lack a significant cytoplasmic component containing antioxidants. Therefore, they are susceptible to lipid peroxidation by the action of the reactive oxygen species (ROS). | Vitamin E and gluthathion as antioxidant in liquid preservation of semen: A review