The present study was conducted in cattle brought to Large Animal Medicine Unit, Teaching Veterinary Clinical Campus, Rajiv Gandhi Institute of Veterinary Education and Research and Ambulatory clinic for treatment of various medical ailments from June 2017 to November 2017. Seventy three cases with clinical signs of pale to icteric mucous membrane and tick infestation were subjected for blood smear examination and Polymerase Chain Reaction (PCR). | Prevalence of anaplasmosis caused by Anaplasma marginale in cattle in and around puducherry region, India