The low cost alternative components of tissue culture media were evaluated for their potential to supply nutrients and to support growing explant of Banana cultivar in vitro for their cost effectiveness against conventional high cost media. The Murashigeand Skoog (MS) salts and sucrose were replaced by vermicompost (50 g/L), table sugar (30 g/L) and coconut water (70 ml/L), and agar was replaced by filter paper, sand and wheat floor. The conventional MS medium supplemented with 30 g/L sucrose and 8 g/L agar was used as control.
