Entotomopathogenic nematodes (Steinernema sp.) isolated from the soil samples in the Barak Valley of Assam, India. The nematodes were isolated by baiting technique using greater wax moth (Galleria mellonella). Soil samples were collected during rabbi and monsoon season from the different locality of Barak Valley especially from crop fields, rubber plantation, pineapple garden, tea gardens and also from rhizospheric soil of bamboo bushes. Nematodes are isolated from rhizospheric soil of Bamboo and from crop field. Only on the basis of morphological characters isolates resembles with the Steinernema sp. | Isolation and microscopic investigation of entomopathogenic nematodes (EPNs) occurring in Barak valley, Assam, India