An attempt was made to find out natural and cheaper methods for the control of vegetable pest. Locally available plant C. procera was used and a good methodology was developed to extract the secondary metabolic compounds from the latex. Secondary metabolic compounds extracted in form of different polarity fractions and in purity of >90% as single pure compound in each fraction, showed tremendous activity in vitro test against secondary stage juveniles of Meloidogyne javanica. The extracted fractions were found toxic to juveniles by different capabilities between concentrations ranged from 50-300 g ml-1 . The activity of compounds was found dependent on concentration, time of exposure and the polarity of the extracted fractions. | Isolation of biocidal compounds of calotropis latex and their in vitro and in vivo effect on the mortality of root knot nematode (Meloidogyne javanica) in Brinjal and Chilli
