Aflatoxins are potent carcinogenic and mutagenic metabolites mainly produced by Aspergillus flavus. A study was conducted to manage the aflatoxin production in maize grains in ambient storage conditions using bioagents ., Trichoderma asperellm, T. viride and Aspergillus niger isolated from maize grain itself and initially tested for antagonistic ability against radial growth of Aspergillus flavus. T. asperellum was found most effective in suppressing the growth of test fungi. | Biological control of aflatoxin in maize grain at ambient storage conditions