V. jatamansi Jones is an important medicinal wild herb of North Eastern Himalayan Region belonging to family Valerianaceae. The herb is widely used in treatment of leprosy, epilepsy, hysteria and asthma and also in making perfumed powder. Due to overexploitation of roots and rhizomes, V. jatamansi is included in endangered species as per National Medicinal Plant Board, New Delhi, India. The harvest of roots and rhizomes causes destruction of whole plant at its natural habitat. An alternative and efficient protocol was developed for in vitro root mass multiplication from leaf explant in order to promote in situ conservation of the herb. | In vitro direct rhizogenesis of an endangered medicinal herb: Valeriana jatamansi Jones