The macaúba palm is a novel feedstock for oil production suitable for multiple uses, including as biodiesel and in the food and cosmetic industries. As an efficient alternative, the macaúba palm has limited genomic resources, particularly expressed sequence tag (EST) markers. | Development of novel EST-SSR markers in the macaúba palm Acrocomia aculeata using transcriptome sequencing and crossspecies transferability in Arecaceae species