In flowering plants, lipid biosynthesis and transport within anthers is essential for male reproductive success. TaMs1, a dominant wheat fertility gene located on chromosome 4BS, has been previously fine mapped and identified to encode a glycosylphosphatidylinositol (GPI)-anchored non-specific lipid transfer protein (nsLTP). | Nội dung Text Wheat TaMs1 is a glycosylphosphatidylinositol-anchored lipid transfer protein necessary for pollen development .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Kouidri et al. BMC Plant Biology 2018 18 332 lt br gt https s12870-018-1557-1 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Wheat TaMs1 is a lt br gt glycosylphosphatidylinositol-anchored lipid lt br gt transfer protein necessary for pollen lt br gt development lt br gt Allan Kouidri1 Ute Baumann1 Takashi Okada1 Mathieu Baes1 2 Elise J. Tucker1 2 and Ryan Whitford1 lt br gt lt br gt lt br gt Abstract lt br gt Background In flowering plants lipid biosynthesis and transport within anthers is essential for male reproductive lt br gt success. TaMs1 a dominant wheat fertility gene located on chromosome 4BS has been previously fine mapped and lt br gt identified to encode a glycosylphosphatidylinositol GPI -anchored non-specific lipid transfer protein nsLTP . Although lt br gt this gene is critical for pollen exine development details of its function remains poorly understood. lt br gt Results In this study we report that TaMs1 is only expressed from the B sub-genome with highest transcript lt br gt abundance detected in anthers containing microspores undergoing pre-meiosis through to meiosis. β-glucuronidase lt br gt transcriptional fusions further revealed that TaMs1 is expressed throughout all anther cell-types. TaMs1 was identified to lt br gt be expressed at an earlier stage of anther development relative to genes reported to be necessary for sporopollenin lt br gt precursor biosynthesis. In anthers missing a functional TaMs1 ms1c deletion mutant these same genes were not lt br gt observed to be mis-regulated indicating an independent function for TaMs1 in pollen .
