Na+ extrusion from cells is important for plant growth in high saline environments. SOS1 (salt overly sensitive 1), an Na+ /H+ antiporter located in the plasma membrane (PM), functions in toxic Na+ extrusion from cells using energy from an electrochemical proton gradient produced by a PM-localized H+ -ATPase (AHA). | Co-expression of SpSOS1 and SpAHA1 in transgenic Arabidopsis plants improves salinity tolerance .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Fan et al. BMC Plant Biology 2019 19 74 lt br gt https s12870-019-1680-7 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Co-expression of SpSOS1 and SpAHA1 in lt br gt transgenic Arabidopsis plants improves lt br gt salinity tolerance lt br gt Yafei Fan1 Xiaochang Yin1 Qing Xie1 Youquan Xia1 Zhenyu Wang1 Jie Song2 Yang Zhou1 and Xingyu Jiang1 lt br gt lt br gt lt br gt Abstract lt br gt Background Na extrusion from cells is important for plant growth in high saline environments. SOS1 salt overly lt br gt sensitive 1 an Na H antiporter located in the plasma membrane PM functions in toxic Na extrusion from cells lt br gt using energy from an electrochemical proton gradient produced by a PM-localized H -ATPase AHA . Therefore lt br gt SOS1 and AHA are involved in plant adaption to salt stress. lt br gt Results In this study the genes encoding SOS1 and AHA from the halophyte Sesuvium portulacastrum SpSOS1 and lt br gt SpAHA1 respectively were introduced together or singly into Arabidopsis plants. The results indicated that either lt br gt SpSOS1 or SpAHA1 conferred salt tolerance to transgenic plants and as expected Arabidopsis plants expressing both lt br gt SpSOS1 and SpAHA1 grew better under salt stress than plants expressing only SpSOS1 or SpAHA1. In response to NaCl lt br gt treatment Na and H in the roots of plants transformed with SpSOS1 or SpAHA1 effluxed faster than wild-type WT lt br gt plant roots. Furthermore roots co-expressing SpSOS1 and SpAHA1 had higher Na and H efflux rates than single lt br gt SpSOS1 SpAHA1-expressing transgenic plants resulting in the former amassing less Na than .
