Plant glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the reduction of dihydroxyacetone phosphate (DHAP) to produce glycerol-3-phosphate (G-3-P), and plays a key role in glycerolipid metabolism as well as stress responses. | A cytosolic NAD -dependent GPDH from maize ZmGPDH1 is involved in conferring salt and osmotic stress tolerance .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Zhao et al. BMC Plant Biology 2019 19 16 lt br gt https s12870-018-1597-6 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt A cytosolic NAD -dependent GPDH from lt br gt maize ZmGPDH1 is involved in conferring lt br gt salt and osmotic stress tolerance lt br gt Ying Zhao1 Meng Liu1 Lin He1 Xin Li2 Feng Wang1 Bowei Yan1 Jinpeng Wei1 Changjiang Zhao1 lt br gt Zuotong Li1 and Jingyu Xu1 lt br gt lt br gt lt br gt Abstract lt br gt Background Plant glycerol-3-phosphate dehydrogenase GPDH catalyzes the reduction of dihydroxyacetone lt br gt phosphate DHAP to produce glycerol-3-phosphate G-3-P and plays a key role in glycerolipid metabolism as well lt br gt as stress responses. lt br gt Results In this study we report the cloning enzymatic and physiological characterization of a cytosolic NAD - lt br gt dependent GPDH from maize. The prokaryotic expression of ZmGPDH1 in showed that the enzyme encoded lt br gt by ZmGPDH1 was capable of catalyzing the reduction of DHAP in the presence of NADH. The functional lt br gt complementation analysis revealed that ZmGPDH1 was able to restore the production of glycerol-3-phosphate and lt br gt glycerol in AtGPDHc-deficient mutants. Furthermore overexpression of ZmGPDH1 remarkably enhanced the lt br gt tolerance of Arabidopsis to salinity osmotic stress by enhancing the glycerol production the antioxidant enzymes lt br gt activities SOD CAT APX and by maintaining the cellular redox homeostasis NADH NAD ASA DHA GSH GSSG . lt br gt ZmGPDH1 OE Arabidopsis plants also exhibited reduced leaf water loss and stomatal aperture under salt and lt br gt .
