Total recovery of fermentable sugar from lignocellulosic biomass has foremost problem for liquid biofuel production. Several pretreatment methods have been tried, however use of ionic liquids is one of promising method then others, but it also has less efficiency. Hence a method was developed for pretreatment of sugarcane leaves using ionic liquid EmimAc (1-ethyl-3-methylimidazolium acetate), aqueous ammonia and a combination of both. The combined use of ionic liquid and aqueous ammonia in 1:1 for pretreatment at 6 hrs followed by cellulase treatment showed a synergistic effect on sugarcane leaves with release of 90% glucose. | Enhanced saccharification in sugarcane leaves by combined pretreatment of ionic liquid and aqueous ammonia
