The present investigation entitled “Optimization Protocol of DNA isolation and PCR in Muskmelon (Cucumis melo L.) by RAPD Marker” was carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (.), 431001. Six different commercial verities of muskmelon plant were studied for this research. | Optimization protocol of DNA isolation and PCR in Muskmelon Cucumis melo L. by RAPD marker