One hundred cattle-calves irrespective of their age, sex and breed brought to Teaching Veterinary Clinical Complex, College of Veterinary and Animal Science, Bikaner were screened for Theileria annulata infection. Blood and lymph node aspirate smears were prepared from the ear vein and enlarged superficial lymph nodes of suspected cattle-calves and stained with Giemsa’s stain for detection of piroplams and schizonts under oil immersion, respectively. | Hospital-prevalence of theileria annulata infection in cattle-calves determined by blood smear and lymph node aspirate smear examination in Bikaner Rajasthan India