This study focused on a) determining spatial and temporal counts of bacteria using the Most Probable Number method (MPN), and b) evaluating the findings in terms of quality parameters of the sediment, sediment pore water and overlying water. Accordingly, surface sediment and water samples were taken at two stations located in areas of the pond, fed by groundwater polluted by agricultural runoff, with and without macrophytes in January, April, July and October 2017. | Evaluation of bacteria abundance and sediment-water quality parameters in a spring originated shallow pond sediment