In present investigation, 133genotypes comprised of advanced mutant breeding lines, germplasm lines, genotypes pertaining to Initial Varietal Trial, Advanced varietal Trial and Multi Location Trial, popular varieties national, zonal and local checks were evaluated for assessment of genetic variability at Regional Agricultural Research Station, Jagtial with Randomized Block design in 3 replications. Influence on breeding programmes is especially based on the extent to which variability occurs and the relative amount of variation in the different traits. | Assessment of genetic variability heritability and genetic gain in advanced mutant breeding lines of sesame Sesamum indicum L.