An experiment was carried out at the Agricultural Research Station, Perumallapalle for the regeneration of somaclones. Two sugarcane clones, 2008T42 (susceptible to red rot) and 2009T5 (susceptible to smut) were used as plant material. Calli from the controls and different concentrations of mutagenic chemicals (sodium nitrite and EMS) were transferred on to MS media with 3 mg l-1 BAP, 2 mg l-1 IAA and 2 mg l-1 kinetin for evaluating the effect on shooting. | Regeneration of sugarcane Saccharum officinarum L. clones from mutagenic treated calli
