RNA sequencing (RNA-seq)-based whole transcriptome analysis (WTA) using ever-evolving nextgeneration sequencing technologies has become a primary tool for coding and/or noncoding transcriptome profiling. As WTA requires RNA-seq data for both coding and noncoding RNAs, one key step for obtaining high-quality RNA-seq data is to remove ribosomal RNAs, which can be accomplished by using various commercial kits. Nonetheless, an ideal rRNA removal method should be efficient, user-friendly and cost-effective so it can be adapted for homemade RNA-seq library construction. | Nội dung Text A reverse transcriptase-mediated ribosomal RNA depletion RTR2D strategy for the cost-effective construction of RNA sequencing libraries
