The genetic polymorphism revealed by biochemical markers (protein and isozymes) in plant population have been extensively used to, assess the genetic variability, confirm hybridity of F1s, assure purity of cultivar and establish relationship between species. Electrophoretic protein analysis holds potential utility in breeding programmes of wheat through helping in the unequivocal characterization of varieties used. Sixteen wheat genotypes along with their 32 F1’s were characterized by using protein profile polymorphism in seeds through SDS-PAGE. | Diversity analysis and hybridity test of drought responsive wheat cultivars and their f1 s on the basis of SDS-PAGE profiles