Pseudomonas fluorescens isolates NLR-B1, NLR-B2, NLR-B3 were tested in dual culture against R. solani among which NLR-B3 isolate was found to be more antagonistic in vitro against Rhizoctonia solani than other two isolates. Sclerotial germination was tested after incubating in NLR-B3 bacterial suspension in the concentrations from 10-4 to 10-8 CFU/ml, there was no inhibition in sclerotial germination at 10 min incubation at all concentrations of bacterial suspension, whereas at 20 min incubation, inhibition of sclerotial germination was per cent at 10-4 concentration, per cent at 10-5 concentration, 30 per cent at 10-6 concentration and there was no inhibition of sclerotial germination at 10-7 and 10-8 concentrations. | Antagonistic effect of pseudomonas fluorescens on the mycelial growth and the viability of the sclerotia of rhizoctonia solani in vitro and in soil
