A brief study was carried on forty Staphylococcus aureus isolates from Bovine milk from Ludhiana, Punjab, India for detecting the antibiotic resistance profile using E-strips and genotypically targeting various antibiotic resistance genes. The antibiotics used for phenotypic resistance detection were Penicillin, Gentamicin, Erythromycin, Clindamycin, Tetracycline, Chloramphenicol, Ciprofloxacin, Trimethoprim-sulphamethoxazole, Oxacillin, Vancomycin and Amoxyclav. The resistance genes targeted were blaZ (Penicillin); ermA, ermB, ermC (Erythromycin); tetK, tetL, tet M, tetO (Tetracycline); aacA-aphD (Gentamycin), mecA (Oxacillin). This study provides a bird view of antimicrobial susceptibility pattern of Staphylococcus aureus in Ludhiana and can help to form scientific basis for field Veterinarians to make therapy decisions empirically thus forming a pillar in combating the antibiotic resistance problem. | Phenotypic and genotypic characterization of antibiotic resistant staphylococcus aureus in bovine milk samples in Ludhiana Punjab India