Vibrio species are the major food borne pathogens commonly associated with aquatic food poisonings and lead to food-borne outbreaks. In this present study, out of 105 fresh water fish (Catlacatla) samples collected, 87 () were found positive for Vibrio species. Out of 87, 6 (), 2 (), 4 () and 3 () isolates were found to be V. parahaemolyticus and V. vulnificus, V. alginolyticus and V. cholerae respectively by mPCR. The 15 different Vibrio species were subjected to antibiogram studies including ESBL detection. | Isolation molecular characterization and antimicrobial resistance patterns of four different vibrio species isolated from fresh water fishes