Azo dyes are commonly used in many commercial industries. 16 bacterial isolates were isolated from textile effluents, of which 4 isolates (HB1, HB2, HB3 and HB4) showed ability to decolorize Trypan blue dye. Based on the standard morphological and biochemical characteristics, HB3 isolate that showed maximum decolorization of Trypan blue was identified as Bacillus sp. HB3 isolate showed % decolorization of Trypan blue within 24 h of incubation. Maximum decolorization of Trypan blue was found to be achieved at 35 °C, neutral pH in the presence of glucose (Carbon source) and Yeast extract (Nitrogen source). | Bioremediation and detoxification of trypan blue by bacillus sp. isolated from textile effluents