Provision of nutrients in balanced form and required amount is crucial to meet the productive targets in dairy cows. Among the various nutrients, protein is the most important nutrient for milk production. Methionine and lysine are considered as the first and second limiting amino acids for milk protein synthesis because of their low concentrations in feed protein as compared to their concentrations in milk and ruminally synthesized bacterial protein. Choline, a component of phospholipid and methyl donor, plays an essential role in biosynthesis and secretion of milk. | Methionine lysine and choline in dairy cows A review article