Bacillus subtilis a gram positive, endospore forming bacteria play a major role in biocontrol and PGPR activities. Thirty isolates of B. subtilis were obtained from different rhizosphere soil samples from different parts of North Eastern Karnataka region. All the isolates were rod shaped, positive for gram reaction, endospore, oxidase, catalase, starch hydrolysis, negative for indole, KOH test and green coloured colonies were grown on Hichrome Bacillus agar medium. | In vitro screening of bacillus subtilis isolates against sclerotium rolfsii cause for collar rot of chilli