Protozoa are unicellular parasites which infect a number of animals including human. For diagnosis of protozoa recent research has been focused almost exclusively on molecular based techniques for the identification and quantification of parasite DNA in samples. The development of molecular tools has allowed the diagnosis, as well as the study of the genetic variability of pathogens and the identification of species-specific markers. Paper review details about the recent advance technique in important protozoan parasite in animal. | Advances in diagnosis of important protozoan diseases Old and new approaches