An experiment was carried out to study the effect of different holding solution combinations on vase life of cut carnation cv. Kiro. The flowers were kept under common holding solution (sucrose 4% + 8-HQS 200 ppm) along with GA3 at 25 ppm recorded the highest chlorophyll content of leaf ( SPAD unit) on 2nd day and gradually decreased through vase life extends, whereas significantly the highest chlorophyll content of calyx ( SPAD unit) extended through the vase life period resulting in reduced microbial count ( × 10-5 cfu/ml) initially and ( × 10-5 cfu/ml) final microbial count thereby extending maximum vase life ( days) which has been extended the vase life period of carnation flower cv. Kiro. | Effect of holding solutions on biochemical and microbial observations in extending the vase life of cut carnation cv. Kiro